WebMar 14, 2016 · For new protein synthesis analysis and imaging, we used Click-iT Plus OPP Alexa Fluor 594 Protein Synthesis Assay Kit (Life Technologies, Carlsbad, CA). In this assay, OPP (O-propargyl-puromycin) is efficiently incorporated into newly translated proteins that can be detected by fluorescently labeled Alexa Fluor dye. One microliter of OPP ... WebFigure 1 Using Click-iT ® Plus OPP to monitor the inhibition of the protein synthesis. The dose-dependent decrease was monitored using either the Alexa Fluor 488 (Figure 1A) …

GSK3β regulates AKT-induced central nervous system axon …

WebClick-iT® Plus OPP Alexa Fluor® Protein Synthesis Assay in Embryonic Cells. Full-text available. Article. Jun 2024 ... WebMotorbike Simulator. 3328519 Plays. With Pop It Plus, the hit of the season, a sensory anti-stress toy pop it is here again! But this time it has some different features! There may be … modifications in a lesson plan

GSK3β regulates AKT-induced central nervous system axon ... - eLife

WebSep 10, 2024 · Prepare the Click-it OPP reaction cocktail following the Click-iT™ Plus OPP Alexa Fluor™ 488 Protein Synthesis Assay Kit from Thermo Fisher Scientific. Prepare all … WebClick-iT® Plus OPP Alexa Fluor® Protein Synthesis Assay in Embryonic Cells. Bio-protocol 12(11): 4441. DOI: 10.21769/BioProtoc.4441. 3 . Published: Jun 05, 2024 2–6 h. (B) Example of OPP detection procedures working with 60- … WebClick+ is a small remote button that helps you complete a variety of tasks with a simple click. Connect the Click+ to your phone via Bluetooth and use the Click+ app to program … modifications for reading fluency