WebUse ZE-Series QC Beads to check that the optics and fluidics the ZE5 Cell Analyzer are performing to specification. Quality control beads for flow cytometry (3–3.4 μm diameters) Fluorescent dyes emitting from ultraviolet to far red. Mixture of single-peak beads matching all fluorescence channels of the ZE5 Cell Analyzer. Web2. Click QC & Setup from the Get started menu. 3. Select the current bead lot number from the Bead Lot menu. 4. Click Start. 5. When Daily QC successfully completes, click View Report to see the Daily QC report. If QC fails, follow the guidelines in the Daily QC Failed dialog that appears. Data Acquisition Click Acquisition from the Get started ...
[Cytometry] CytoFLEX QC beads
WebCytek Biosciences Web4 Cytek 6-Color TBNK-SL Reagent Instructions for Use R9-52000US Rev. A • Vortex mixer • Flow cytometer (Cytek® Northern Lights™-CLC) • SpectroFlo® QC Beads 6. Specimen Requirements 1 Require peripheral blood of no less than 500µL collected by venipuncture in EDTA anticoagulant tube. Refer to manufacturer’s instructions for ... smart alkaline battery charger
Cytek AURORA Operation Protocol.pdf - Course Hero
WebThe gains pre-loaded in the software are derived from the “Cytek Assay Settings” which are based on the QC beads and are calculated on a daily basis during the quality control process. Cytek Assay Settings are a universal baseline for instrument performance to facilitate stability and normalization across instruments and time. Web9. Verify that the 2 bead populations within the beads A and B gates are on scale on the R4 axis in the R4 vs. YG1/B4 plots found in the downloaded LEGENDplex™ Experiment Template . a. If your Cytek® instrument does not have a YG laser: b. If your Cytek® instrument has a YG laser: 10. Vortex the vial of LEGENDplex™ Setup Beads 2: PE … WebSetting Up an Experiment on the Cytek Aurora Emory Pediatrics/Winship Flow Cytometry Core Version: 1.02 Updated: 22 March 2024 i. If you will be running some single stained controls on beads and others on cells, check the ‘Define Additional Negative ontrols’ box and add an additional negative control and choose its type. ii. hill and clark holbeach