WebOct 11, 2024 · However, to our surprise, shLuc exerted severe off-target effects on voltage-gated ion channel function, while the shScr had no detectable changes. These results indicate that thorough validation of shRNA is imperative and, in the absence of such validation, that shScr is the best available negative control for gene knockdown studies. WebPlasmid pLKO.1 shSCR from Dr. Sheila Stewart's lab contains the insert shSCR and is published in Curr Biol. 2008 Apr 8. 18(7):496-500. This plasmid is available through …
【レディース】 BIGOTRE ビゴターバッグ IO4ih-m52899887625
WebViable cell density was determined by absorbance measurement following solubilization of the dye. Representative images of tumor cell colonies at day 6 of culture are shown. (B) shScr-, shLyn1-, or shLyn2-transduced BlgCre Brca1 fl/fl p53 +/− tumor cells (1–3) seeded in Matrigel (3D) were assessed for growth after 6 days. WebNov 11, 2024 · The dashed line presents FAO in shScr at baseline. n=4. All data are presented as mean+SEM. P values were determined by unpaired Student t test (A and B) or 1-way ANOVA followed by Tukey multiple comparison test (D–G). *P<0.05 vs shScr; #P<0.05 vs shScr+PE. shACC2 indicates short hairpin RNA against ACC2; and shScr, scrambled … chi st vincent heart center hot springs ar
pLKO.1 shSCR Addgene 17920 product information
WebOur coordinator Mrs. Sarwanti Purwandari Jalan Bukit Golf L2/No. 1, Citraland Citraland Surabaya East Java,East Java 60211 WebHepatic ChREBP knockdown reduces downstream enzymes of glycolysis and increases hepatic G6P and glycogen storage in L-G6pc −/− mice.(A) Box-and-whisker plots presenting relative hepatic mRNA levels of Chrebpα and Chrebpβ and relative protein abundance of G6PC and ChREBP in L-G6pc +/+ and L-G6pc −/− mice treated with either shChREBP or … WebFeb 17, 2024 · (A and B) Confocal analysis of PINK1 accumulation and PARK2 recruitment at mitochondria upon CCCP treatment in shSCR and shBECN1 SH-SY5Y cells, overexpressing PINK1-GFP (A) or YFP-PARK2 (B). Cells were treated with DMSO alone or with 25 μM CCCP for 3 h and mitochondria were immunostained using the mitochondrial marker TOMM20 … chi st vincent hot springs cardiology